An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction

M Negahdary, M Behjati-Ardakani, N Sattarahmady, H Heli

Abstract


Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.

Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.

Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor.

Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively.

Conclusions: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.


Keywords


Electrochemical Biosensor, Biomarker, Heart failure, Troponin, Molecular Detection

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DOI: https://doi.org/10.22086/jbpe.v0i0.930

eISSN: 2251-7200        JBPE NLM ID: 101589641

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